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Documenti fondamentali

CMC0016

Sigma-Aldrich

BL21(DE3) Electrocompetent Cells

Escherichia coli, rod shaped

Sinonimo/i:

BL21 strain

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About This Item

Codice UNSPSC:
41106202
NACRES:
NA.85

Nome del prodotto

BL21(DE3) Electrocompetent Cells, for protein expression

Origine biologica

Escherichia coli

Grado

Molecular Biology
for molecular biology

Modalità di accrescimento

adherent or suspension

Morfologia

rod shaped

tecniche

microbiological culture: suitable

Trasformazione cellulare

competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Descrizione generale

The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Caratteristiche e vantaggi

The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 109 cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment

Componenti

  • BL21(DE3) electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Codice della classe di stoccaggio

10 - Combustible liquids

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Certificati d'analisi (COA)

Lot/Batch Number
SLCM4783
SLCP1550
SLCM4784
SLCJ4196
SLCJ5997

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Julianne M Troiano et al.
eLife, 10 (2021-01-16)
Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as
Felix Nicolaus et al.
eLife, 10 (2021-02-09)
We follow the cotranslational biosynthesis of three multispanning Escherichia coli inner membrane proteins in vivo using high-resolution force profile analysis. The force profiles show that the nascent chain is subjected to rapidly varying pulling forces during translation and reveal unexpected
Salvatore Di Girolamo et al.
Microbial cell factories, 19(1), 170-170 (2020-08-29)
Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows

Protocolli

BL21(DE3) Electrocompetent Cells

BL21(DE3) Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one reaction. Transformation is carried out in a 0.1 cm gap cuvette. Optimal settings for electroporation are listed in the table below. Note that alternate settings result in transformation efficienes about 20-50% lower. Typical time constants are 3.5 to 4.5 msec.

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