CLLS1003

DLD1 Cells PIK3CA +/-

human male colon (Source Disease: Colorectal adenocarcinoma)

• Codice UNSPSC: 41106514
• NACRES: NA.81

Proprietà

• product name: DLD1 Cells PIK3CA +/-,
• Origine biologica: human male colon (Source Disease: Colorectal adenocarcinoma)
• N° accesso OMIM: 171834
• Temperatura di conservazione: −196°C
• Informazioni sul gene: human ... PIK3CA(5290)

Descrizione

Descrizione generale

DLD1 Cells PIK3CA +/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN knock out modification.

Applicazioni

Media Renewal changes two to three times per week

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3 to 1:10

The base medium for this cell line is RPMI, Cat. No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%.

The cell freezing medium is DMSO 1X (Cat. No. C6164)

Caratteristiche e vantaggi

These DLD-1 cells are adherent, with a doubling time of approx. 20 hours.

Zinc finger nuclease (ZFN) knock out on chromosome 3q23.3

Qualità

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

I componenti del kit sono disponibili anche separatamente

• DLD1 Control Cells 1 vial
• DLD1 Cells PIK3CA +/- 1 vial

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable