Nb2-11 Cell Line from rat

Thymus/lymph node, lymphoblast morphology, 97041101

• Codice UNSPSC: 41106514
• eCl@ss: 32190102

Proprietà

• Origine biologica: rat lymph
• Modalità di accrescimento: Suspension
• Cariotipo: 42XY,-17,t(2,4)(q22,q24),t(10,11)(q12,q12),+der(15)t(15,?)(p12,?),-18,+der(18)t(18,?)(p12,?)
• Morfologia: Lymphoblast
• Prodotti: Not specified
• Recettori: Modified prolactin receptor (the Nb2 form)
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat lymphoma

Descrizione della linea cellulare

Nb2-11 is a clone of the Nb-2 rat lymphoma line which was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Nb2-11 can also be mitogenically stimulated by IL-2. Injection of Nb2 cells into Nb rats gives rise to malignant tumours that are highly sensitive to treatment with vinca alkaloids. Karyotypic analysis has shown that the cell line has only five well developed chromosome abnormalities. The cells do not express surface immunoglobulin, and their lactogen dependency was confirmed before deposit. Protocols for the use of Nb2-11 cells in bioassays are available from ECACC on request.

Applicazioni

1) in vitro bioassay of lactogenic hormones.
2) Studies of the mechanisms of lactogen induced mitogenesis.
3) Studies into the action of vinca alkaloids.
4) Studies of tumour progression.

Terreno di coltura

Fischer′s medium + 10% Fotal Bovine Serum (FBS) + 10% Horse Serum (HS) (gelding) + 0.075% Sodium Bicarbonate + 0.05 mM 2-Mercaptoethanol (2ME) + 2 mM Glutamine.

Mantenimento delle subcolture

At resuscitation seed cultures at 3-5 x 100,000 cells/ml. Once established cultures should be seeded at 4 x 1,000 and 12 x 1,000 cells/ml for culture periods of 96 and 72 hours respectively. Do not exceed 9 x 100,000 cells/ml. Avoid using dilutions of cells with fresh medium at greater than 1:10 ratios; culture doubling time 12 hours.

Altre note

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