GP5d
95090715, human colon, Epithelial
Sinonimo/i:
GP5d Cells
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About This Item
Prodotti consigliati
product name
GP5d, 95090715
Origine biologica
human colon
Modalità di accrescimento
Adherent
Cariotipo
Diploid
Morfologia
Epithelial
Prodotti
Not specified
Recettori
Epidermal growth factor (EGF)
tecniche
cell culture | mammalian: suitable
Malattie correlate
cancer
Condizioni di spedizione
dry ice
Temperatura di conservazione
−196°C
Origine della linea cellulare
Human Caucasian colon adenocarcinoma
Descrizione della linea cellulare
GP5d has been established from the same adenocarcinoma as GP2d (Sigma Catalogue number. 95090714). This has been confirmed by STR profiling at ECACC. The cells were derived from a local recurrence of Duke′s grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo- or radio therapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess changes consistent with the usual pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP5d cells express relatively more TGF α mRNA than GP2d cells. GP5d cells undergo a marked epithelial-mesemchymal transition when treated with EGF.
Applicazioni
Colon tumour assays, influence of growth factors and adhesion molecules on colonic tumour cells
Terreno di coltura
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Mantenimento delle subcolture
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 5x10,000 to 1x100,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Altre note
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