SK-N-BE(2)

95011815, human nerve, Epitheilioid & neuroblastoid

• Codice UNSPSC: 41106514

Proprietà

• product name: SK-N-BE(2), 95011815
• Origine biologica: human nerve
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 44
• Morfologia: Epitheilioid & neuroblastoid
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: metastasis
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian neuroblastoma

Descrizione della linea cellulare

Established from a bone marrow biopsy of a 22-month-old male with disseminated neuroblastoma in 1972. The morphology varies with sub-population from cells with long processes through small, flattened, epithelioid cells. Dense cultures tend to form loosely adherent aggregates. They are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine.

Applicazioni

Chemotherapy development, chromosome studies, drug resistance, differentiation studies.

Terreno di coltura

EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham′s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:10 to 1:25 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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