RC-4B/C1

94020810, rat brain, Epithelial-like

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: RC-4B/C1, 94020810
• Origine biologica: rat brain
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial-like
• Prodotti: Gonadatrophs
• Recettori: Gonadotrophin-releasing hormone receptors
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat pituitary adenoma

Descrizione della linea cellulare

RC-4B/C1 is a clone of the parent RC-4B/C which was established from a 3 year-old CAI/FMaixF344/FMai(F1) male rat pituitary adenoma. Staining studies have shown the cell line contains many bi-hormonal cells with FHSß, LHß and prolactin. Electron microscopy shows the appearance of endogenous C-type rat retroviral particles.

Applicazioni

Study of gonadotrophic functions of pituitary gland and regulation of pituitary gonadotrophin gene expression.

Terreno di coltura

DMEM:EMEM (EBSS) (1:1) + 2mM Glutamine + 0.2mg/ml BSA + 0.5% Non Essential Amino Acids (NEAA) + 5% Nu-Serum (Collab)+ 2ng/ml EGF.

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:6 i.e. seeding at 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 10% CO₂; 34°C. Saturation density is reached at 1x 100,000 cells/cm². Cells may attach lightly and so handling should be kept to a minimum. Care shoul

Altre note

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