h1RPE7

9061602, human eye, Polygonal

• Codice UNSPSC: 41106514

Proprietà

• product name: h1RPE7, 09061602, from human eye
• Origine biologica: human eye
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Polygonal
• Prodotti: Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice

Descrizione

Origine della linea cellulare

An immortalized retinal pigment epithelial cell line

Descrizione della linea cellulare

The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.

Profilo DNA

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19

Terreno di coltura

Hams F10 + 20% Heat-inactivated FCS + 2 mM Glutamine + selection antibiotic 1μg/ml puromycin.

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 24hrs.

Altre note

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Esclusione di responsabilità

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.