M3 Clone M-3

93120827, mouse skin, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: M3 Clone M-3, 93120827
• Origine biologica: mouse skin
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 83; hypertetraploid
• Morfologia: Epithelial
• Prodotti: Melanin
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse Cloudman S 91 melanoma

Descrizione della linea cellulare

Melanin producing cell line, derived from a Cloudman S 91 melanoma induced in a F1 (CxDBA) male mouse. The line is tumourigenic in mice and susceptible to a variety of viruses including herpes simplex, vaccinia, pseudorabies and vesicular stomatitis virus (Indiana virus).

Applicazioni

Tumourigenicity studies

Terreno di coltura

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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