TM3

91060526, mouse testis, Not specified

• Codice UNSPSC: 41106514

Proprietà

• product name: TM3, 91060526
• Origine biologica: mouse testis
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Not specified
• Prodotti: Prostaglandin F2a
• Recettori: Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse BALB/c testis, Leydig cell

Descrizione della linea cellulare

Originated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic.

Applicazioni

Hormone studies

Terreno di coltura

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37²C.

Altre note

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