LL24

90073001, human lung, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: LL24, 90073001
• Origine biologica: human lung
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 46; diploid
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human diploid lung

Descrizione della linea cellulare

Derived from normal tissue taken at post-mortem from the lung of a 5 year old male. An ampoule of cells frozen after one passage and estimated population doubling (PDL) 2 was submitted to the American Type Culture Collection in June, 1977. Derived populations at passage 6 and approximately 14 PDL are available for distribution. Progeny were found to undergo an additional 25 population doublings in longevity trials.

Applicazioni

Tumourigenicity studies

Terreno di coltura

Ham′s F12 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, a further 20 population doublings expected.

Altre note

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