LA-4

90040512, mouse lung, Epithelial-like

• Codice UNSPSC: 41106514

Proprietà

• product name: LA-4, 90040512
• Origine biologica: mouse lung
• Modalità di accrescimento: Adherent
• Cariotipo: Unstable, range 38-256 chromosomes
• Morfologia: Epithelial-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse A/He lung adenoma

Descrizione della linea cellulare

Isolated from a urethan-induced lung adenoma of a 28 week old A/He strain mouse. The cells incorporate high levels of 14C-choline into lecithin. C-type particles have been observed in some cultures.

Applicazioni

Tumourigenicity studies

Terreno di coltura

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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