UT-1

87100701, hamster ovary, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: UT-1, 87100701
• Origine biologica: hamster ovary
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 22
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Hamster Chinese ovary

Descrizione della linea cellulare

Derived from parent line CHO-K1. Cells were exposed to increasing concentrations of compactin over 8 months. During this exposure the level of 3-hydroxy-3-methylglutaryl coenzyme A reductase synthesis increased 500-fold. The enzyme controls cholesterol synthesis.

Applicazioni

Enzymatic studies

Terreno di coltura

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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