HT 1376

87032402, human bladder, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: HT 1376, 87032402
• Origine biologica: human bladder
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 46, hypertetrapoid
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian bladder carcinoma

Descrizione della linea cellulare

Derived from a 58 year old caucasian female with a grade 3 transitional carcinoma of the bladder who had not received chemo or radiation therapy. The cells grow in soft agar and are tumorigenic in mice and hamsters. The patient donor had not received chemotherapy or radiation treatment.

Applicazioni

Tumourigenicity studies

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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