COS-7 Cell Line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87021302, African green monkey kidney, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: African green monkey kidney
• Descrizione: Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• Confezionamento: tube of 5 μg 87021302-DNA-5UG; pkg of vial of cells 87021302-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Monkey African green kidney, SV40 transformed

Descrizione della linea cellulare

Derived from CV-1 simian cells transformed by an origin-defective mutant of SV40. The cells support the growth of recombinant SV40 viruses. The cells should be handled at laboratory containment level 2.

Applicazioni

COS-7 Cell Line has been used to:

• test the functional role of ankyrin B in ouabain/Na,K-ATPase/1,4,5-trisphosphate receptor (IP3R) signaling
• study the expression of green fluorescent protein-tagged constructs of neuropathy target esterase (NTE) and mutant proteins lacking the transmembrane, catalytic and regulatory domains
• study the distribution of peroxisomes

Virus replication studies: SV40

Terreno di coltura

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)..

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.