GH3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012603, rat brain, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: rat brain
• Confezionamento: tube of 5 μg 87012603-DNA-5UG; pkg of vial of cells 87012603-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Hyperdiploid, modal no. 60
• Morfologia: Epithelial
• Prodotti: Growth hormone, prolactin
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat Wistar-Furth pituitary tumour

Descrizione della linea cellulare

Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.

Applicazioni

Hormone studies

Terreno di coltura

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask

Altre note

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