KG-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86111306, human bone marrow, Myeloblast-like

• Sinonimo/i: KG1 Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human bone marrow
• Confezionamento: tube of 5 μg 86111306-DNA-5UG; pkg of vial of cells 86111306-1VL
• Modalità di accrescimento: Suspension
• Cariotipo: 2n = 46, diploid
• Morfologia: Myeloblast-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian bone marrow myelogenous leukaemia

Descrizione della linea cellulare

Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.

Applicazioni

KG-1 has been used to extract total RNA and use them as templates to determine the 5′ end of promyelocytic leukemia zinc finger (PLZF) transcripts.

Profilo DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

Terreno di coltura

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Altre note

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