AC2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse blood
• Confezionamento: tube of 5 μg 86061902-DNA-5UG; pkg of vial of cells 86061902-1VL
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Lymphoblast
• Prodotti: Not specified
• Recettori: Interleukin 3 (IL-3)
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse lymphoblast

Descrizione della linea cellulare

Derived from a mouse basophil clone and is dependent on Interleukin-3 (IL-3) for growth. IL-3 may be added in the form of Wehi conditioned media (10-20%). Culture densities between 10,000 and 100,000 cells/ml is necessary to retain cells in exponential growth phase.

Applicazioni

Growth factor studies, IL-2 assays

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO₂; 37°C. Cells will rapidly die at high densities above 5x100,000 cells/ml. Culture very sensitive to agitation.

Altre note

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