P3HR-1

86010701, human lymph, Lymphoblast

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: P3HR-1, 86010701
• Origine biologica: human lymph
• Confezionamento: tube of 5 μg 86010701-DNA-5UG; pkg of vial of cells 86010701-1VL
• Modalità di accrescimento: Suspension
• Cariotipo: 2n = 46, hyperdiploid
• Morfologia: Lymphoblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Burkitt′s Lymphoma

Descrizione della linea cellulare

Clonally-derived sub-line of JIYOYE (Sigma Catalogue number. 88071302) containing high levels of EBV. Has been successfully hybridised with the human lymphoid cell line K562 to form the hybrid PUTKO-1. ACDP class II pathogen and should be handled under laboratory containment level 2. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.