BRL 3A

85111503, rat liver, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: BRL 3A, 85111503
• Origine biologica: rat liver
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: MSA factors
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Buffalo Rat liver

Descrizione della linea cellulare

Originally isolated by primary cloning. Serum-free medium conditioned by the cells produces a family of polypeptides termed multiplication stimulating activity (MSA).

Applicazioni

Growth factor studies

Terreno di coltura

Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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