BHK 21 (Clone 13) from hamster

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011433, hamster kidney, Fibroblast

• Sinonimo/i: BHK-21 (C-13) Cells, BHK-21 C-13 Cells, BHK21 clone 13 Cells, BHK21-C13 Cells, BHK21/C13 Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: hamster kidney
• Confezionamento: tube of 5 μg 85011433-DNA-5UG; pkg of vial of cells 85011433-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 44, pseudodiploid
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Hamster Syrian kidney

Descrizione della linea cellulare

Subclone of parent line derived from 5 1-day-old unsexed hamster kidneys. Used extensively for virus replication studies i.e. poliovirus, rabies, foot and mouth disease, VSV (Indiana strain), herpes simplex, Ad25 and arboviruses.

Applicazioni

BHK 21 (Clone 13) from hamster has been used in viral transfection studies.

Virus replication studies

Terreno di coltura

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 5-10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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