MRC-5 PD 19 Cell Line human

5072101, human lung, Fibroblast-like

• Sinonimo/i: MRC-V Cells, MRC5 Cells, Medical Research Council Cell Strain-5
• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: MRC-5 PD 19 Cell Line human, 05072101
• Origine biologica: human lung
• Descrizione: Human fetal lung
• Modalità di accrescimento: Adherent
• Cariotipo: 2n=46, diploid
• Morfologia: Fibroblast-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human foetal lung

Descrizione della linea cellulare

Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.

Applicazioni

It has been used as a cell substrate to study the production of recombinant MVA (modified vaccinia virus Ankara).

Profilo DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum FBS / FCS.

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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