ANGM-CSS

8040401, human brain, Fusiform, can form heaps of cells

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: ANGM-CSS, 08040401
• Origine biologica: human brain
• Modalità di accrescimento: Not specified
• Cariotipo: Near tetraploid; range 88 to 91 - see Cell Line Description for more information
• Morfologia: Fusiform, can form heaps of cells
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human glioblastoma

Descrizione della linea cellulare

A human A human glioblastoma multiforme cell line derived from a 65-year-old male. The results of immunocytochemical analysis for GFAP, Vimentin, NestiN and CD33 were positive in early and late passage cells. MGMT methylation was negative. Karyotype: Near tetraploid; range 88 to 91 with two copies of marker: der(6;7;12;)del(6q ), t(6;11), t(6;17) del(17q). Six copies of chromosome 7 and other rearrangements with loss and gain of chromosome fragments involving chromosomes 1,2,3,5,10,9,19,20. The X and Y chromosomes are present. (Information provided by Depositor)

Terreno di coltura

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂;37ºC.

Altre note

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