SNL 76/7 Cell Line from mouse

7032801, mouse esophagus, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: SNL 76/7 Cell Line from mouse, 07032801
• Origine biologica: mouse esophagus
• Descrizione: Mouse SIM strain embryonic fibroblast
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse SIM strain embryonic fibroblast

Descrizione della linea cellulare

SNL 76/7 clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF). The STO cell line was transfected with a G418-resistance cassette, RV4.0 (Thomas and Capecchi 1987), and a LIF expression construct (Williams et al., 1988). This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells. Inclusion of G418 in the media is not necessary for normal cell growth.

Terreno di coltura

Knockout D-MEM (Invitrogen) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Mantenimento delle subcolture

Split confluent cultures 1:5-1:15 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;5% CO₂; 37°C.

Altre note

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