Huh-7D 12 Cell Line human

1042712, human liver, Epithelial

• Sinonimo/i: Huh7 Cells, JTC-39 Cells, Japanese Tissue Culture-39 Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: Huh-7D 12 Cell Line human, 01042712
• Origine biologica: human liver
• Modalità di accrescimento: Adherent
• Cariotipo: That of original Huh-7
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Temperatura di conservazione: −196°C

Descrizione

Descrizione generale

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.

Origine della linea cellulare

Human hepatocellular carcinoma

Descrizione della linea cellulare

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.

Applicazioni

Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18

Terreno di coltura

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 C. Media change twice a week

Altre note

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