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Key Documents

C-22210

Sigma-Aldrich

Endothelial Cell Growth Medium

Basal Medium, 500 ml

Sinonimo/i:

Primary Cell Culture Media, Recommended with C-39215 or C-39210

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.71

Forma fisica

aqueous solution

Confezionamento

pkg of 500 ml

tecniche

cell culture | mammalian: suitable

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Descrizione generale

Endothelial Cell Basal Medium (supplements sold separately)

To form a complete medium, it should be combined with the media supplements: C-39215 or C-39210

Applicazioni

Endothelial Cell Growth Medium is a low-serum (2% V/V) medium optimized for the cultivation of endothelial cells from large blood vessels. The media are optimized for primary human cells but can also be used for bovine, porcine, murine, caprine, rabbit, elephant, dog, and rat endothelial cells from large vessels as well as for various endothelial cell lines.Note: For best transfection results we recommend using PromoCell heparin-free Endothelial Cell Growth Medium MV2. Heparin, included all our other our Endothelial Cell Growth Media formulations, may reduce transfection efficiency.

Componenti

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualità

All lots of PromoCell Endothelial Cell Media are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Stoccaggio e stabilità

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Mantenimento delle subcolture

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1


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Protocolli

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

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