B5388

Brilliant Cresyl Blue ALD

Dye content, ≥60%, certified by the Biological Stain Commission, powder

• Sinonimo/i: 3-Amino-7-(diethylamino)-2-methylphenoxazin-5-ium (T-4)-tetrachlorozincate (2:1), Brilliant Blue C, Brilliant Cresyl blue
• Formula condensata: C₁₇H₂₀N₃OCl · 1/2ZnCl₂
• Numero CAS: 51716-96-2
• Peso molecolare: 385.96
• Colour Index: 51010
• Beilstein: 5690713
• Numero CE: 279-675-0
• Numero MDL: MFCD00148901
• Codice UNSPSC: 12171500
• ID PubChem: 24891836
• NACRES: NA.47

Proprietà

• product name: Brilliant Cresyl Blue ALD, certified by the Biological Stain Commission
• Grado: certified by the Biological Stain Commission
• Forma fisica: powder
• Composizione: Dye content, ≥60%
• Solubilità: water: 1 mg/mL, blue to very deep blue
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: room temp
• InChI: 1S/2C17H20N3O.4ClH.Zn/c2*1-4-20(5-2)12-6-7-14-16(9-12)21-17-10-13(18)11(3)8-15(17)19-14;;;;;/h2*6-10H,4-5,18H2,1-3H3;4*1H;/q2*+1;;;;;+2/p-4; SIHASHFMDFXGSX-UHFFFAOYSA-J

Descrizione

Applicazioni

Brilliant Cresyl Blue ALD has been used for the selection of oocytes. It has also been used for the selection of cumulus-oocyte complexes (COCs).

Azioni biochim/fisiol

Brilliant Cresyl Blue ALD is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD). This enzyme is generated in growing oocytes. However, oocytes which complete their growth have low levels of G6PD. Low G6PD activity results in blue coloration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound. It is also used for the staining in reticulocytes, where the dye stains reticulin.

Compatibilità

Certified as a supravital stain for reticulocytes.

Altre note

The structure of Brilliant Cresyl Blue ALD contains a 2-methyl modification on the phenoxazine core, while the structure of the historic Brilliant Cresyl Blue contains a 8-methyl modification on the phenoxazine core. The biological staining application is the same for both structures.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves