A9542
Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Sinonimo/i:
Rabbit Anti-Rat IgG (whole molecule)−HRP
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About This Item
Numero MDL:
Codice UNSPSC:
12352203
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Coniugato
peroxidase conjugate
Forma dell’anticorpo
IgG fraction of antiserum
Tipo di anticorpo
secondary antibodies
Clone
polyclonal
Forma fisica
buffered aqueous solution
Reattività contro le specie
rat
tecniche
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500
western blot (chemiluminescent): 1:5,000 using detecting rat primary antibody
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
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Categorie correlate
Descrizione generale
IgG (Immunoglobulin G) antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulins have two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein which mainly helps in immune defense. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
Specificità
Anti-Rat IgG (whole molecule) antibody recognizes rat (immunoglobulin G) IgG and is determined by immunoelectrophoresis (IEP) versus normal rat serum and rat IgG prior to conjugation.
Immunogeno
purified rat IgG
Applicazioni
Anti-Rat IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in western blotting and enzyme linked immunosorbent assay (ELISA).
Azioni biochim/fisiol
IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Nota sulla preparazione
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Stoccaggio e stabilità
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1 - Skin Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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