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A8111

Sigma-Aldrich

Plasminogen activator inhibitor 1 (PAI-1)

≥200,000 units/mg protein, lyophilized powder, ≥90% (SDS-PAGE)

Sinonimo/i:

PAIE, PLANH1, Plasminogen activator inhibitor, β-migrating endothelial cell-derived type, Serpin E1 PAI-1, Serpine 1

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About This Item

Numero CAS:
140208-23-7
Numero MDL:
MFCD01094930
Codice UNSPSC:
12352200
NACRES:
NA.32

product name

Plasminogen activator inhibitor 1 (PAI-1) human, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Ricombinante

expressed in E. coli

Livello qualitativo

200

Saggio

≥90% (SDS-PAGE)

Forma fisica

lyophilized powder

Attività specifica

≥200,000 units/mg protein

PM

~43 kDa

Solubilità

water: 0.1 mL, clear, colorless

N° accesso UniProt

P05121

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... SERPINE1(5054)

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Descrizione generale

Plasminogen activator inhibitor 1 (PAI-1) or serine protease inhibitor E1 (SERPINE1) gene is mapped to human chromosome 7q22.1. PAI-1 is produced in hepatocytes, endothelial cells, smooth muscle cells, megakaryocytes, and adipocytes.

Applicazioni

Plasminogen activator inhibitor 1 (PAI-1) human has been used:
  • as an inhibitor of pro-brain-derived neurotrophic factor (proBDNF) in radioimmunoprecipitation assay (RIPA) in peripheral blood and lymphocytes
  • to block plasmin activation in mice infected with C. albicans hyphae
  • to test its effect on neurite growth in neurons

Azioni biochim/fisiol

Plasminogen Activator Inhibitor 1 (PAI-1) protein belongs to the Serpin superfamily of serine protease inhibitors. It is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and therefore inhibits the fibrinolysis process. PAI is involved in extracellular matrix remodeling and has been implicated in processes like angiogenesis, chemotaxis, ovulation, and embryogenesis. PAI-1 is present in increased levels in various disease states such as a number of cancer forms, obesity and the metabolic syndrome.
Plasminogen activator inhibitor–1 (PAI -1) participates in mediating cellular senescence. It is upregulated during injury along with other tissue repair transcriptomes. Elevated levels of PAI-1 is regarded as one of the risks associated with myocardial infarction (MI).

Definizione di unità

1 unit irreversibly inhibits (100% inhibition) 1 unit of tissue plasminogen activator (tPA) under physiological conditions (neutral pH and 37 ºC).

Stato fisico

Supplied as a lyophilized powder from a solution containing 0.5 M sodium chloride, 20 mM sodium acetate, and 50 mg/mL trehalose.

Ricostituzione

Reconstitute to 0.25 mg/ml with ultrapure water. The reconstituted product contains 0.25 mgP/mL in 0.5 M sodium chloride, 20 mM sodium acetate, and 50 mg/mL trehalose.

Risultati analitici

The product is the active human PAI-1 protein with a molecular weight of 43 kDa.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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William Santus et al.
Science immunology, 2(15) (2017-09-25)
Nuclear factor of activated T cells (NFAT) is activated in innate immune cells downstream of pattern recognition receptors, but little is known about NFAT's functions in innate immunity compared with adaptive immunity. We show that early activation of NFAT balances
Bo-Yang Yu et al.
Biology of reproduction, 100(6), 1473-1481 (2019-04-03)
Plasminogen activator, tissue type (PLAT) and its inhibitor serpin family E member 1 (SERPINE1) cooperatively regulate PLAT activity in various reproductive processes. However, it is unknown whether this includes bovine oocyte maturation. We addressed this question in the present study
Natália Salazar et al.
Research in microbiology, 168(2), 157-164 (2016-12-19)
A previous study had demonstrated that Leptospira enolase is secreted extracellularly by a yet unknown mechanism and reassociates with the bacterial membrane. Surface-anchored leptospiral enolase displays plasminogen binding activity. In this work, we explored the consequences of this interaction and
Winfried Neuhaus et al.
Frontiers in molecular neuroscience, 10, 149-149 (2017-06-13)
The blood-brain barrier (BBB) is damaged during ischemic insults such as traumatic brain injury or stroke. This contributes to vasogenic edema formation and deteriorate disease outcomes. Enormous efforts are pursued to understand underlying mechanisms of ischemic insults and develop novel

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