A6941
Alcohol Oxidase from Candida boidinii
lyophilized powder, 5-15 units/mg protein
Sinonimo/i:
AOD1, AOX, Alcohol:oxygen oxidoreductase
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About This Item
Prodotti consigliati
Origine biologica
fungus (Candida boidinii)
Livello qualitativo
Forma fisica
lyophilized powder
Attività specifica
5-15 units/mg protein
PM
octomer 600 kDa by sedimentation equilibrium
Solubilità
100 mM potassium phosphate, pH 7.5: soluble 1.0 mg/mL at 25 °C (Cold)
Temperatura di conservazione
−20°C
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Descrizione generale
Research area: Cell Signaling
Alcohol Oxidase (AOX) is a homo-octamer composed of eight flavin adenine dinucleotide (FAD) cofactors and belongs to the glucose-methanol-choline (GMC) family of oxidoreductases. The AOX1 and AOX2 genes are responsible for encoding AOX. Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm. Alcohol oxidase is a 600 kDa homooctomeric flavoprotein with eight equal 74 kDa subunits; each containing a flavin adenine dinucleotide (FAD) molecule.
Alcohol Oxidase (AOX) is a homo-octamer composed of eight flavin adenine dinucleotide (FAD) cofactors and belongs to the glucose-methanol-choline (GMC) family of oxidoreductases. The AOX1 and AOX2 genes are responsible for encoding AOX. Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm. Alcohol oxidase is a 600 kDa homooctomeric flavoprotein with eight equal 74 kDa subunits; each containing a flavin adenine dinucleotide (FAD) molecule.
Applicazioni
Alcohol oxidase has been used:
- to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes .
- to study methanol metabolism in yeasts, such as Candida, Pichia, and Hansenula.
- to study protein translocation into peroxisomes.
- for the determination of ethanol concentration in alcoholic drinks using enzymatic assay.
- in development of enzyme electrode for the determination of alcohols.
Azioni biochim/fisiol
Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. The enzyme shows little activity toward secondary, tertiary, or aromatic alcohols; or aliphatic alcohols with a chain length of more than 5 carbons. The pH range for activity of this product is 6.5-8.5, with the optimum pH being 7.5. Alcohol oxidases facilitate the conversion of alcohol into carbonyl compounds while producing hydrogen peroxide. It is also the first enzyme in the yeast methanol utilization pathway.
Definizione di unità
One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25 °C.
Stato fisico
Contains potassium phosphate buffer salts, DTE, and stabilizer
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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I clienti hanno visto anche
Alcohol oxidase from Candida boidinii.
H Sahm et al.
Methods in enzymology, 89 Pt D, 424-428 (1982-01-01)
B Vinet
Clinical chemistry, 33(12), 2204-2208 (1987-12-01)
This method for the specific determination of methanol in serum is based on the following two reactions: (formula; see text) Alcohol oxidase is not specific: it converts all lower alcohols to their corresponding aldehydes; however, formaldehyde dehydrogenase is specific and
Yu Sasano et al.
FEMS yeast research, 10(5), 535-544 (2010-05-25)
We identified a gene, designated TRM2, responsible for methanol-inducible gene expression in the methylotrophic yeast Candida boidinii. The encoded protein Trm2p contains two C(2)H(2)-type zinc finger motifs near the N terminus and shows high similarity to Saccharomyces cerevisiae Adr1p and
Ping Zhang et al.
Applied and environmental microbiology, 76(18), 6108-6118 (2010-07-27)
In this work, the identification and characterization of two hexose transporter homologs in the methylotrophic yeast Pichia pastoris, P. pastoris Hxt1 (PpHxt1) and PpHxt2, are described. When expressed in a Saccharomyces cerevisiae hxt-null mutant strain that is unable to take
Yasutaka Sasaki et al.
Journal of bioscience and bioengineering, 110(2), 147-151 (2010-06-16)
We have cloned a gene encoding an alcohol oxidase (AOD) specific to aldehyde alcohols from Paenibacillus sp. AIU 311. The AOD gene contains an open reading frame consisting of 618 nucleotides corresponding to 205 amino acid residues. The deduced amino
Protocolli
This procedure may be used for all Alcohol Oxidase products.
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