A3429

Differentiation Solution

for the differentiation of regressive hematoxylin stains

• Sinonimo/i: Acid Alcohol
• Numero MDL: MFCD00243254
• Codice UNSPSC: 12171500
• NACRES: NA.47

Proprietà

• Stato: solution
• Durata: 40 mo.
• Colore: colorless
• applicazioni: hematology; histology
• Temperatura di conservazione: room temp

Descrizione

Descrizione generale

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolorize hematoxylin stain. The duration of exposure to the solution determines the degree of decolorization. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper color contrast necessary for visualization, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

Applicazioni

Acidified alcohol solution for the differentiation of regressive hematoxylin stains.

Differentiation solution is employed mainly for the differentiation of regressive hematoxylin stains. It has been used in the following studies:

• the prediction of pulmonary metastasis progression in osteosarcoma patients
• the development of multispectral imaging to detect melanin

Caratteristiche e vantaggi

• Desired staining patterns can be obtained by varying the degree of exposure to the differentiation solution.
• Results are sharper and more rapid compared to other differentiation methods.
• Crisp and clear differentiation between cellular structures is obtainable when performed according to Sigma-Aldrich Procedure No. GHS.

Principio

In regressive hematoxylin staining, a highly concentrated hematoxylin solution is used to rapidly overstain both nuclei and cytoplasm, and the cytoplasm is subsequently decolorized and excess hematoxylin is removed from the nucleus with dilute acid. Improper differentiation leaves excess residual hematoxylin that obscures the fine details of nuclear structures and chromatin and prevents eosin uptake.

Informazioni sulla sicurezza

• Pittogrammi: GHS02,GHS08,GHS05
• Avvertenze: Danger
• Indicazioni di pericolo: H225,H290,H319,H371
• Consigli di prudenza: P210 - P233 - P234 - P240 - P305 + P351 + P338 - P308 + P311
• Classi di pericolo: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 2
• Organi bersaglio: Eyes,Central nervous system
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): 71.6 °F
• Punto d’infiammabilità (°C): 22 °C