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A3219

Sigma-Aldrich

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)

peroxidase substrate, chromogenic, liquid

Sinonimo/i:

ABTS

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About This Item

Numero MDL:
MFCD00214141
Codice UNSPSC:
12352204
ID PubChem:
24890730
NACRES:
NA.83

Nome del prodotto

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Liquid Substrate System

Livello qualitativo

200

Stato

liquid

Temperatura di conservazione

2-8°C

Stringa SMILE

CCN1C(\Sc2cc(ccc12)S(O)(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S(O)(=O)=O

InChI

1S/C18H18N4O6S4/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28)/b19-17-,20-18-
ZTOJFFHGPLIVKC-CLFAGFIQSA-N

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Descrizione generale

The product is supplied as a ready-to-use one component substrate for peroxidase consisting 2,2′-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid) in an acidic buffer. The substrate is colorless before reacting with peroxidase and produces a blue-green color reaction product after reacting with it. 1% sodium dodecyl sulfate (SDS) may be used to stop the reaction during end-point assays. Since this substrate produces a soluble reaction product, it is not recommended for immunoblotting or histochemical applications.

Applicazioni

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) has been used as a peroxidase substrate in ELISA experiment. This substrate gives a green color soluble end product. It has also been used as a colloidal preparation spread over the standard media plates, to evaluate the lignin oxidation activity. Presence of green coloration of the substrate exhibits microbial laccase activity.

Azioni biochim/fisiol

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a cation and undergoes oxidation in the presence of hydrogen peroxide. The oxidized ABTS is a blue-green product and is measurable colorimetrically. A combination of ABTS, hydrogen peroxide and myoglobin is a standard system employed for measuring total antioxidant capacity(TAC) from samples. ABTS method is flexible at varying pH and most widely used. ABTS is a substrate for phenol oxidase enzyme.

Stato fisico

Ready-to-use.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
MKCQ1056
MKCM9916
MKCC7644
MKCP2423
MKCG9831

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Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis
Shalaby EA and Shanab SMM
Indian Journal of Geo-Marine Sciences, 42(5), 556-564 (2013)
Randall L Davis et al.
Journal of neuroimmunology, 186(1-2), 141-149 (2007-05-04)
Emerging evidence indicates that neuroinflammatory responses in astroglia, including chemokine expression, are altered by opioids. Astroglial chemokines, such as CXCL10, are instrumental in response to many neuropathological insults. Opioid mediated disruption of astroglial CXCL10 expression may be detrimental in opioid
A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation
Erel O
Clinical Biochemistry, 37(4), 277-285 (2004)
Yehoshua Gozes et al.
Infection and immunity, 74(2), 1266-1272 (2006-01-24)
Bacillus anthracis lethal toxin (LT) is a bipartite toxin composed of protective antigen (PA) and lethal factor (LF). Injection of LT produces clinical signs characteristic of anthrax infection, including pleural edema and vascular collapse in various animal models. We utilized
Efficient assay for total antioxidant capacity in human plasma using a 96-well microplate
Kambayashi Y, et al.
Journal of Clinical Biochemistry and Nutrition, 44(1), 46-51 (2009)
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Articoli

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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