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Key Documents

93773

Sigma-Aldrich

TWEEN® 20

viscosity 250-450 mPa.s (25 °C)

Sinonimo/i:

Monolaurato di polietilenglicole sorbitano, Monolaurato di poliossietilene sorbitano

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About This Item

Numero CAS:
Numero MDL:
Codice UNSPSC:
12161900
NACRES:
NA.21

Descrizione

non-ionic

Forma fisica

viscous liquid

PM

~1228

tecniche

DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable

Indice di rifrazione

n20/D 1.468 (lit.)
n20/D 1.471

Viscosità

250-450 mPa.s(25 °C)

HLB

16.7±1.0

Densità

1.11 g/mL at 20 °C
1.095 g/mL at 25 °C (lit.)

Stringa SMILE

CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
HMFKFHLTUCJZJO-UHFFFAOYSA-N

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Descrizione generale

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Applicazioni

Tween® 20 is widely used in several biochemical applications. It has been used:

  • as an emulsifying agent for the preparation of stable oil-in-water emulsions
  • in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
  • as a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%
  • for lysing mammalian cells at a concentration of 0.005 to 0.5%
  • for rehydrating tissue before in situ hybridization
Detergente non ionico

Azioni biochim/fisiol

Tween® 20, functioning as a detergent, potentially operates by binding to the hydrophobic components of water-insoluble substances, thereby making them hydrophilic.

Caratteristiche e vantaggi

  • Suitable for protein purification and DNA and RNA extraction
  • Suitable for mammalian cell culture
  • High purity product for research applications

Avvertenza

may get turbid on storage, heat to clear

Altre note

For additional information on our range of Biochemicals, please complete this form.

Note legali

TWEEN is a registered trademark of Croda International PLC

Prodotto comparabile

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

527.0 °F - Pensky-Martens closed cup

Punto d’infiammabilità (°C)

275 °C - Pensky-Martens closed cup

Dispositivi di protezione individuale

Eyeshields, Gloves


Certificati d'analisi (COA)

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