83702

Rhodamine 123

BioReagent, for fluorescence, ≥85% (HPLC)

• Sinonimo/i: 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester
• Formula condensata: C₂₁H₁₇ClN₂O₃
• Numero CAS: 62669-70-9
• Peso molecolare: 380.82
• Beilstein: 6030951
• Numero CE: 263-687-8
• Numero MDL: MFCD00012664
• Codice UNSPSC: 12352116
• ID PubChem: 57652966
• NACRES: NA.32

Proprietà

• Grado: for fluorescence
• Nome Commerciale: BioReagent
• Saggio: ≥85% (HPLC)
• Stato: powder
• Impurezze: ≤10% water
• Solubilità: ethanol: 20 mg/mL, clear, red
• Compatibilità: in accordance for fluorescence
• Stringa SMILE: Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24
• InChI: 1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H; MYFATKRONKHHQL-UHFFFAOYSA-N

Descrizione

Descrizione generale

Rhodamine 123 is a fluorescent dye.

Applicazioni

Rhodamine 123 has been used:

• as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
• to label the cells for estimating the P-gp expression by flow cytometry
• in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
• the detection of mitochondrial membrane potential (ΔΨm)

Azioni biochim/fisiol

Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.

Altre note

Supravital mitochondrial-specific dye

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)