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73737

Sigma-Aldrich

4-Nitrophenyl phosphate bis(tris) salt

phosphatase substrate, ≥97.0% (enzymatic), powder or crystals

Sinonimo/i:

p-Nitrophenylphosphate bis(tris) salt, p-nitrophenyl phosphate bis(tris) salt, PNPP, PNPP bis(tris) salt

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About This Item

Formula condensata:
(C6 H4 N O6 P)( C4 H12 N O3)2
Numero CAS:
68189-42-4
Peso molecolare:
461.36
Beilstein:
1578142
Numero CE:
269-198-6
Numero MDL:
MFCD00037009
Codice UNSPSC:
12352204
ID PubChem:
57652488
NACRES:
NA.32

product name

4-Nitrophenyl phosphate bis(tris) salt, for the determination of phosphatase, ≥97.0% (enzymatic)

Saggio

≥97.0% (enzymatic)

Forma fisica

powder or crystals

Qualità

for the determination of phosphatase

Impurezze

≤0.1% free nitrophenol
≤6% water

Solubilità

H2O: 0.1 g/mL, clear

Temperatura di conservazione

2-8°C

Stringa SMILE

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
XXAXKCWOTRABOW-UHFFFAOYSA-N

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Applicazioni

4-Nitrophenyl phosphate bis(tris) salt has been used to determine the enzyme activity of acid and alkaline phosphatase in cell lysates.

Azioni biochim/fisiol

4-Nitrophenyl phosphate is a non-proteinaceous and non-specific chromogenic substrate, preferred for alkaline phosphatase (ALP) in enzyme-linked immunosorbent assay (ELISA). It is widely used to assay protein tyrosine phosphatases (PTPs). Upon cleavage, the substrate produces a yellow product that is spectrophotometrically measured.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Elif Özlem Arslan-Aydoğdu et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 49(1), 189-199 (2017-10-19)
Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase
D.W. Moss
Methods of Enzymatic Analysis, 92-92 null
Aneta Pietraszek et al.
Colloids and surfaces. B, Biointerfaces, 173, 1-8 (2018-09-28)
Alkaline phosphatase (ALP) was sucessfully incorporated into the halloysite (HAL) nanotubes, as confirmed by FTIR-ATR and XRD analyses. The loading efficiency (LE) of ALP was found to be 13.5%, while its encapsulation efficiency (EE) was estimated to be around 27%
O.H. Lowry
Methods in Enzymology, 4, 366-366 (1957)
Mary E Wood et al.
Vaccine, 36(50), 7737-7743 (2018-11-12)
Chronic wasting disease (CWD) is a fatal prion disease affecting multiple cervid species. Effective management tools for this disease, particularly in free-ranging populations, are currently limited. We evaluated a novel CWD vaccine in elk (Cervus canadensis) naturally exposed to CWD
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Articoli

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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