71183

Sodium acetate

anhydrous, BioUltra, for luminescence, for molecular biology, ≥99.0% (NT)

• Sinonimo/i: Sodium acetate anhydrous, Acetic acid Sodium salt anhydrous, Acetic acid sodium salt
• Formula condensata: CH₃COONa
• Numero CAS: 127-09-3
• Peso molecolare: 82.03
• Beilstein: 3595639
• Numero CE: 204-823-8
• Numero MDL: MFCD00012459
• Codice UNSPSC: 12161700
• eCl@ss: 39021906
• ID PubChem: 57652226
• NACRES: NA.25

Proprietà

• Grado: for luminescence; for molecular biology
• Livello qualitativo: 200
• Nome Commerciale: BioUltra
• Saggio: ≥99.0% (NT)
• Stato: solid
• Temp. autoaccensione: 1112 °F
• Impurezze: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• Perdita: ≤0.5% loss on drying, 150 °C
• pH: 8.5-10.0 (25 °C, 3 M in H₂O)
• pKa: 4.76 (acetic acid)
• Punto di fusione: >300 °C (dec.) (lit.)
• Solubilità: H₂O: 3 M at 20 °C, clear, colorless
• Anioni in tracce: chloride (Cl^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• Cationi in tracce: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤10 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤200 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤10 mg/kg; Zn: ≤5 mg/kg
• Stringa SMILE: [Na+].CC([O-])=O
• λ: 3 M in H₂O
• Assorbanza UV: λ: 260 nm A_max: 0.03; λ: 280 nm A_max: 0.02
• InChI: 1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1; VMHLLURERBWHNL-UHFFFAOYSA-M

Descrizione

Applicazioni

Sodium acetate is suitable for use as a buffer in:

• protein crystallization
• staining of gels in protein gel, electrophoresis, neutralization buffer for chromatography
• the preparation of standard (acetylcholine chloride) for hestrin assay
• substrate screening characterization for microfluidic microbial fuel cell array

Azioni biochim/fisiol

Sodium acetate anhydrous is commonly used in molecular biology applications. This serves as a buffering agent in the DNA digestion experiments by ensuring the reaction has optimum pH. In general sodium acetate is preferred in purification, precipitation, crystallization, and other applications which require tight control of elemental content. Sodium acetate is extensively used as a retardant in plastics manufacturing, as a mordant in dyeing, and in the tanning of leather.

Altre note

Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation).

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)