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56748

Sigma-Aldrich

Imidazolo

BioUltra, for molecular biology, ≥99.5% (GC)

Sinonimo/i:

1,3-diaza-2,4-ciclopentadiene, Gliossalina

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About This Item

Formula empirica (notazione di Hill):
C3H4N2
Numero CAS:
288-32-4
Peso molecolare:
68.08
Beilstein:
103853
Numero CE:
206-019-2
Numero MDL:
MFCD00005183
Codice UNSPSC:
12352005
eCl@ss:
39161001
ID PubChem:
57651596
NACRES:
NA.25

Grado

for molecular biology

Livello qualitativo

200

Tensione di vapore

<1 mmHg ( 20 °C)

Nome Commerciale

BioUltra

Saggio

≥99.5% (GC)

Forma fisica

flakes
powder or crystals

Impurezze

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
≤0.2% water

Residuo alla calcinazione (900 °C)

≤0.05%

pH

9.5-11.0 (25 °C, 0.1 M in H2O)

pKa (25 °C)

6.95

P. eboll.

256 °C (lit.)

Punto di fusione

88-91 °C (lit.)

Solubilità

H2O: 0.1 M at 20 °C, clear, colorless

Anioni in tracce

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

Cationi in tracce

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

Stringa SMILE

c1c[nH]cn1

λ

0.1 M in H2O

Assorbanza UV

λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.10

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
RAXXELZNTBOGNW-UHFFFAOYSA-N

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Applicazioni

Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.
Ottimo per tamponi nell′intervallo di pH 6,2-7,8

Altre note

Review: As RNase model

Avvertenze

Danger

Indicazioni di pericolo

H302 - H314 - H360D

Classi di pericolo

Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

Codice della classe di stoccaggio

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

293.0 °F - closed cup

Punto d’infiammabilità (°C)

145 °C - closed cup

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Supelco

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Review: As RNase model.
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Methods have been developed for the solid-phase detection of nucleic acids using mixed films of quantum dots (QDs) and oligonucleotide probes in microtiter plates. Polystyrene microwells were functionalized with multidentate imidazole ligands to immobilize QDs. Oligonucleotide hybridization was transduced using
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