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50649

Sigma-Aldrich

Glyoxal solution

for molecular biology, BioReagent, ~40% in H2O (~8.8 M)

Sinonimo/i:

Ethanedial, Oxalaldehyde

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About This Item

Formula condensata:
OHCCHO
Numero CAS:
107-22-2
Peso molecolare:
58.04
Beilstein:
1732463
Numero MDL:
MFCD00006957
Codice UNSPSC:
12352114
ID PubChem:
57651264
NACRES:
NA.52

Grado

for molecular biology

Livello qualitativo

100

Nome Commerciale

BioReagent

Forma fisica

liquid

Concentrazione

~40% in H2O (~8.8 M)

Impurezze

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

Indice di rifrazione

n20/D 1.409

Anioni in tracce

chloride (Cl-): ≤100 mg/kg
sulfate (SO42-): ≤100 mg/kg

Cationi in tracce

Ca: ≤5 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤300 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

Temperatura di conservazione

2-8°C

Stringa SMILE

[H]C(=O)C([H])=O

InChI

1S/C2H2O2/c3-1-2-4/h1-2H
LEQAOMBKQFMDFZ-UHFFFAOYSA-N

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Categorie correlate

Descrizione generale

Glyoxal is a nucleic acid denaturant. It acts as a specific probe for homopurine strands of DNA.

Applicazioni

Suitable for
  • nucleic acid denaturation
  • binding of DNA/RNA to nitrocellulose membrane

Altre note

This form of glyoxal is composed of 3 moles of glyoxal and 2 moles of water in a relatively stable configuration.
May precipitate on storage; redissolve at 50-60 °C.

Avvertenza

May precipitate on storage; redissolve at 50-60 °C.

Principio

Glyoxal forms stable complex with guanine residues that results in denaturation of nucleic acids.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazardExclamation mark
GHS08,GHS07

Avvertenze

Warning

Indicazioni di pericolo

H315,H317,H319,H332,H335,H341

Classi di pericolo

Acute Tox. 4 Inhalation - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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M Vojtisková et al.
Journal of biomolecular structure & dynamics, 5(2), 283-296 (1987-10-01)
Plasmid pEJ4, which is a derivative of pUC19 containing an insert with 60-bp-long homopurine.homopyrimidine tract from sea urchin P. miliaris histone gene spacer, was studied by chemical probes of the DNA structure osmium tetroxide and glyoxal. The former probe reacts
Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
P S Thomas
Methods in enzymology, 100, 255-266 (1983-01-01)
R N Skopp et al.
Analytical biochemistry, 169(1), 132-137 (1988-02-15)
This paper describes agarose gel electrophoresis and silver staining of denatured RNAs. Glyoxal- or formaldehyde-denatured RNAs are electrophoresed in an agarose gel cast on a plastic support using an inert, low conductivity buffer. Following electrophoresis, the gel is stained with
Yu Wang et al.
Chemical Society reviews, 41(11), 4140-4149 (2012-04-18)
Methylglyoxal (MGO) and glyoxal (GO), known as reactive carbonyl species, can be generated endogenously and exogenously (human body and food system). They are attracting increased attention because of their relationship with diabetes and flavour generation. In this review, their characteristics
Fien Van Lancker et al.
Journal of agricultural and food chemistry, 60(18), 4697-4708 (2012-04-03)
Only a minor part of Maillard reaction studies in the literature focused on the reaction between carbohydrates and peptides. Therefore, in continuation of a previous study in which the influence of the peptide C-terminal amino acid was investigated, this study
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