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SFRVN

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Sf-RVN™ Insect Cell Line

Sinonimo/i:

Sf9 rhabdovirus-negative (Sf-RVN™) Insect Cell Line

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About This Item

Descrizione

Sf9 rhabdovirus-negative (Sf-RVN™) Insect Cell Line

Solubilità

DMSO: 10%

Condizioni di spedizione

liquid nitrogen

Temperatura di conservazione

−196°C

Descrizione generale

Sf-RVN(R)cells are provided to customers in vials containing 1 mL at 10 x 106 cells/mL. Cells are banked in EX-CELL® CD Insect Cell Medium and 10% DMSO.
The Sf-RVN™ Insect Cell Line is a rhabdovirus-negative Sf9 cell line that can be grown in both adherent and suspension culture. Sf-RVN™ Insect Cell Line has been adapted for growth in chemically defined medium to be used for the production of recombinant proteins, adeno-associated viruses (AAV) and viral-like particles (VLP). The cell line was tested for adventitious agents and banked under GMP conditions. A technical user guide, with detailed protocols for optimal performances, is provided with the cell line.The EX-CELL® CD Insect Cell Medium, recommended for use, is optimized to get excellent growth and productivity of the Sf-RVN™ Insect Cell Line. Combined, these two products form the Sf-RVN™ Platform.

Cell Line Origin

Sf-RVN™ is an insect cell line originally derived from ovarian tissue of Spodoptera frugiperda (Sf) pupae. The parental line, IPLB-SF-21 (renamed IPLB-SF-21 AE after adaptation into TC-100 medium), was established by J. Vaughn at the Insect Pathology Beltsville in Maryland, USA in the late 1960s. A subclone, designated Sf9, was established in 1983 by G. Smith and C. Cherry. Sf-RVN™ was isolated from Sf9 by GlycoBac, LLC.

Stato fisico

Sf-RVN™ cells are provided to customers in vials containing 1 mL at 10 x 106cells/mL. Cells are banked in EX-CELL® CD Insect Cell Medium and 10% DMSO

Altre note

Sf-RVN™ is an insect cell line originally derived from ovarian tissue of Spodoptera frugiperda (Sf) pupae. The parental line, IPLB-SF-21 (renamed IPLB-SF-21 AE after adaptation into TC-100 medium), was established by J. Vaughn at the Insect Pathology Beltsville in Maryland, USA in the late 1960s. A subclone, designated Sf9, was established in 1983 by G. Smith and C. Cherry. Sf-RVN ™ was isolated from Sf9 by GlycoBac, LLC.

Note legali

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
EXACT-AIR is a registered trademark of Merck KGaA, Darmstadt, Germany
Sf-RVN is a trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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