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Documenti fondamentali

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E2028

Sigma-Aldrich

Ethidium bromide monoazide

≥95% purity (HPLC), solid

Sinonimo/i:

3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide, Ethidium monoazide bromide

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About This Item

Formula empirica (notazione di Hill):
C21H18BrN5
Numero CAS:
58880-05-0
Peso molecolare:
420.31
Numero MDL:
MFCD01708677
Codice UNSPSC:
12171500
ID PubChem:
329799025
NACRES:
NA.47

product name

Ethidium bromide monoazide, ≥95% (HPLC), solid

Livello qualitativo

100

Saggio

≥95% (HPLC)

Forma fisica

solid

tecniche

titration: suitable

Colore

orange

Solubilità

DMF: soluble
ethanol: soluble

ε (coefficiente d’estinzione)

≥5200 at 459-465 nm

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

−20°C

Stringa SMILE

[Br-].CC[n+]1c(-c2ccccc2)c3cc(ccc3c4ccc(N)cc14)N=[N+]=[N-]

InChI

1S/C21H17N5.BrH/c1-2-26-20-12-15(22)8-10-18(20)17-11-9-16(24-25-23)13-19(17)21(26)14-6-4-3-5-7-14;/h3-13,22H,2H2,1H3;1H
GHUXAYLZEGLXDA-UHFFFAOYSA-N

Categorie correlate

Azioni biochim/fisiol

Photoactive stain covalently binds to nucleic acids in solution and in cells with damaged membranes.
Used to footprint drug binding sites on DNA, detect non-viable cells, identify ethidium binding sites on DNA and tRNA, and selectively inactivate gene expression.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
MKCQ2256
MKCM5347
MKCM5140
MKCM2062
MKCK9279

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IGEPAL® CA-630 for molecular biology

Sigma-Aldrich

I8896

IGEPAL® CA-630

G Loozen et al.
Molecular oral microbiology, 26(4), 253-261 (2011-07-07)
DNA-based methodology for the identification and detection of specific bacteria in dental plaque offers advantages over culturing techniques. One drawback of current molecular techniques like real-time quantitative polymerase chain reaction (RT-QPCR) is that they are not able to distinguish between
G Douglas Inglis et al.
Applied and environmental microbiology, 76(4), 1110-1119 (2009-12-22)
The persistence of naturally occurring campylobacteria in aerobic compost constructed of manure from beef cattle that were administered chlortetracycline and sulfamethazine (AS700) or from cattle not administered antibiotics (control) was examined. Although there were no differences in population sizes of
Takashi Soejima et al.
Analytical biochemistry, 418(1), 37-43 (2011-07-21)
The polymerase chain reaction (PCR) can confirm the presence of bacteria, but it is unable to differentiate between live and dead bacteria. Although ethidium monoazide (EMA)- and propidium monoazide (PMA)-based PCR have been evaluated, a quantity of ≥ 10(3)cells/ml dead
N-T Chen et al.
Journal of applied microbiology, 109(2), 623-634 (2010-02-19)
To optimize ethidium monoazide (EMA) coupled with real-time quantitative PCR (qPCR) and to evaluate its environmental applicability on quantifying viable legionellae in water and biofilm of cooling towers and hot water systems. EMA (0.9-45.5 microg ml(-1)) and propidium monoazide (PMA
K Kim et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 63(3), 502-507 (2011-02-01)
A method was developed for discriminating damaged viruses or naked viral RNA from intact viruses by ethidium monoazide (EMA) treatment before RT-PCR. The applied EMA treatment consisted of three steps: (1) EMA dose, (2) exposure to light, and (3) additional
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