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C2734

Sigma-Aldrich

5-Carboxytetramethylrhodamine

≥95% purity (HPLC), solid

Sinonimo/i:

5-TAMRA

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About This Item

Formula empirica (notazione di Hill):
C25H22N2O5
Numero CAS:
91809-66-4
Peso molecolare:
430.45
Numero MDL:
MFCD01073638
Codice UNSPSC:
12171500
NACRES:
NA.47

product name

5-Carboxytetramethylrhodamine,

Saggio

≥95% (HPLC)

Livello qualitativo

100

Forma fisica

solid

Solubilità

water: slightly soluble

ε (coefficiente d’estinzione)

≥85000-97000 at 539-545 nm in methanol

Fluorescenza

λex ~543 nm; λem ~570 nm in methanol

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

InChI

1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
YMZMTOFQCVHHFB-UHFFFAOYSA-N

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Categorie correlate

Descrizione generale

An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

Applicazioni

5-Carboxytetramethylrhodamine has been used in fluorophore characterization.

Azioni biochim/fisiol

5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures. It is mainly used for single-molecule studies. 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
MKCQ1970
MKCM5246
MKCH9058
MKCG8513
MKCF9257

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G Vámosi et al.
Biophysical journal, 71(2), 972-994 (1996-08-01)
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