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C0537

Sigma-Aldrich

5-Carboxyfluorescein

≥99% purity (HPLC), powder

Sinonimo/i:

5-FAM

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About This Item

Formula empirica (notazione di Hill):
C21H12O7
Numero CAS:
76823-03-5
Peso molecolare:
376.32
Beilstein:
57037
Numero MDL:
MFCD00036874
Codice UNSPSC:
12171500
ID PubChem:
24892258
NACRES:
NA.47

product name

5-Carboxyfluorescein, ≥99% (HPLC)

Livello qualitativo

200

Saggio

≥99% (HPLC)

Forma fisica

powder

Solubilità

0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

2-8°C

Stringa SMILE

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
NJYVEMPWNAYQQN-UHFFFAOYSA-N

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Applicazioni

5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.

Azioni biochim/fisiol

5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
088M4144V
116M4127V
13161101
13161101
066M4115V

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Relation between insulin resistance and fast-migrating LDL subfraction as characterized by capillary isotachophoresis.
Zhang B, et al.
Journal of Lipid Research, 46, 2265-2265 (2005)
M Goetz et al.
Endoscopy, 39(4), 350-356 (2007-04-12)
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CD3ac, an uncharged and strongly hydrophobic 10 amino acid peptide (Ac-LK(Ac)-LK(Ac)-LK(Ac)-LW-DL-LW-DL-LW-DL-LW-NH2) was synthesized and purified. The peptide readily dissolves in ethanol and--upon solvent exchange to water--assembles into solid spherical particles with diameters of around 500 nm and low size-polydispersity. CD3ac
Sabina Gerber et al.
The Journal of biological chemistry, 288(13), 8849-8861 (2013-02-06)
N-Linked glycosylation is an essential post-translational protein modification in the eukaryotic cell. The initial transfer of an oligosaccharide from a lipid carrier onto asparagine residues within a consensus sequon is catalyzed by oligosaccharyltransferase (OST). The first X-ray structure of a
Kotyk A and Slavik J
Intracellular pH and its Measurement (1989)
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