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NGLYF-RO

Roche

N-Glycosidase F

PNGase F of Flavobacterium meningosepticum, recombinant from E. coli

Sinonimo/i:

glycosidase

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About This Item

Classificazione EC (Enzyme Commission):
3.5.1.52 (BRENDA, IUBMB)
Codice UNSPSC:
12352204

Ricombinante

expressed in E. coli

Livello qualitativo

100

Coniugato

(N-linked)

Saggio

≥90% (SDS-PAGE)

Forma fisica

solution

Attività specifica

~25000 units/mg protein

Confezionamento

pkg of 0.1 mL (11365169001 [100 U])
pkg of 0.25 mL (11365177001 [250 U])

Produttore/marchio commerciale

Roche

pH ottimale

7.0-8.0

Temperatura di conservazione

−20°C

Categorie correlate

Descrizione generale

Peptide-N-glycosidase F, Peptide-N4-(acetyl-β-glucosaminyl)-asparagine amidase

Applicazioni

Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a lyophilizate without glycerol.

Definizione di unità

One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycopeptide within 1 minute at 37 °C at pH 7.8.

Stato fisico

Solution in 50 mM sodium phosphate, 12.5 mM EDTA, 50% glycerol (v/v), pH 7.2

Altre note

For life science research only. Not for use in diagnostic procedures.

Note legali

The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

No data available

Punto d’infiammabilità (°C)

No data available

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Ewoud van Tricht et al.
Talanta, 144, 1030-1035 (2015-10-11)
Current methods for the identification and/or quantification of viral proteins in influenza virus and virosome samples suffer from long analysis times, limited protein coverage and/or low accuracy and precision. We studied and optimized capillary gel electrophoresis (CGE) in order to
Fabian Higel et al.
mAbs, 6(4), 894-903 (2014-05-23)
N-glycosylation is a complex post-translational modification with potential effects on the efficacy and safety of therapeutic proteins and known influence on the effector function of biopharmaceutical monoclonal antibodies (mAbs). Comprehensive characterization of N-glycosylation is therefore important in biopharmaceutical development. In
Gerard Ja Rouwendal et al.
mAbs, 8(1), 74-86 (2015-10-07)
Monomeric IgA has been proposed as an alternative antibody format for cancer therapy. Here, we present our studies on the production, purification and functional evaluation of anti-HER2 IgA antibodies as anti-cancer agents in comparison to the anti-HER2 IgG1 trastuzumab. MALDI-TOF

Protocolli

N-Glycosidase F Protocol & Troubleshooting

N-Glycosidase F Protocol & Troubleshooting

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