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EXPEXTKB

Roche

KAPA Express Extract

Sinonimo/i:

PCR, PCR extraction

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About This Item

Codice UNSPSC:
41106300
NACRES:
NA.55

Descrizione

for extraction only

Livello qualitativo

impiego

sufficient for 100 reactions
sufficient for 250 reactions
sufficient for 50 reactions
sufficient for 500 reactions

Durata

≤12 mo.

Confezionamento

pkg of 100 U (KK7100)
pkg of 1000 U (KK7103)
pkg of 200 U (KK7101)
pkg of 500 U (KK7102)

Produttore/marchio commerciale

Roche

tecniche

DNA extraction: suitable
PCR: suitable

Temperatura di conservazione

−20°C

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Descrizione generale

KAPA Express Extract is a novel thermostable protease and buffer system that allows for the extraction of polymerase chain reaction (PCR)-ready DNA in as little as 15 minutes. DNA extractions are conveniently performed in a single-tube thereby greatly reducing the risk of sample loss and contamination.
The combination of KAPA Express Extract and KAPA2G Robust HotStart® ReadyMix provides a high performance solution for rapid DNA extraction and consistent downstream amplification.

Applicazioni

KAPA Express Extract is ideally suited for the extraction of polymerase chain reaction (PCR)-ready DNA from, but not limited to, the following sample types:
  • Human tissue (formalin-fixed paraffin-embedded (FFPE) samples; blood collected in EDTA tubes or on collection cards; buccal swabs; hair follicles; forensic samples; biopsies)
  • Animal samples (ear punches; tail clippings; hair follicles; blood; bone marrow; dried or fresh tissue from mouse and other mammals)
  • Fish tissues (fin punches; fresh tissue; cold and hot smoked canned samples; ethanol-preserved samples; embryo)
  • Insects (crushed)
  • Bird feathers (calamus fragments).
  • Nosema species

Caratteristiche e vantaggi

Key Features:
  • Rapid extraction protocol: PCR-ready DNA in 15 minutes.
  • Versatility: single kit optimized for successful extraction from a variety of tissue types, including buccal swabs, hair follicles, fish tissue, and bird feathers.
  • Single-tube reaction minimizes risk of contamination.
  • Produces high amounts of genomic DNA of good quality.
  • DNA extracted with KAPA Express Extract is compatible with any PCR system.

Increased PCR success from formalin-fixed paraffin-embedded (FFPE) samples or tissues:
  • Rapid DNA extraction and consistent downstream amplification of challenging sample types.

Routine DNA extraction from blood sample types:
  • Efficient extraction from “Guthrie”cards, FTA Elute cards, and FTA cards.

Quick Notes:
  • Extract PCR-ready DNA from different tissue types in a quick and simple, single-tube protocol (15–20 min), without the need for hazardous chemicals or multiple washing steps.
  • Significantly reduced turnaround times and risk of sample loss and contamination.
  • Use 1 μL DNA extract per 25 μL PCR. Depending on the sample type, the extract may have to be diluted prior to PCR.
  • One extract typically yields a sufficient amount of template for 50–500 PCRs.
  • DNA extracts may be diluted in TE Buffer (1:5) or 10 mM Tris-HCl pH 8–8.5 (1:5–1:10) for long-term storage at -20°C. Extracts are stable at -20°C for ≥6 months.
  • KAPA2G Robust HotStart® ReadyMix is recommended for consistent and reliable endpoint amplification of DNA extracts generated with KAPA Express Extract.
  • No quantification of DNA required prior to PCR.

Qualità

All components contained with KAPA Express Extract Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activities, and meet strict requirements with respect to DNA contamination.

Nota sulla preparazione

Handling:
KAPA2G Robust HotStart ReadyMix may not freeze solidly, even when stored at -20°C. This will not affect the shelf-life of the product. Always ensure that the product has been fully thawed and mixed before use.

Altre note

For Research Use Only. Not for use in diagnostic procedures.

Note legali

FTA is a trademark of Qiagen Group
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Solo come componenti del kit

N° Catalogo
Descrizione

  • KAPA Express Extract Enzyme 1 U/µL

  • 10X KAPA Express Extract Buffer

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.

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