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10108987001

Roche

Pyrophosphatase, inorganic (PPase)

from yeast

Sinonimo/i:

inorganic pyrophosphatase

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About This Item

Classificazione EC (Enzyme Commission):
Codice UNSPSC:
12352204

Origine biologica

yeast

Livello qualitativo

Forma fisica

suspension

Attività specifica

~200 units/mg protein (At 25 °C with inorganic pyrophosphate as the substrate.)

Confezionamento

pkg of 1 mL (1 mg)

Produttore/marchio commerciale

Roche

Temperatura di conservazione

2-8°C

Descrizione generale

Pyrophosphate phosphohydrolase
The enzyme pyrophosphatase is ubiquitously distributed in a number of organisms.

Specificità

Specific activity: Approximately 200 U/mg at +25°C with inorganic pyrophosphate as the substrate.

Applicazioni

Inorganic pyrophosphatase has been used in enzyme assays and enzyme inhibition studies.
Pyrophosphatase, inorganic (PPase) has been used in :
  • Enzymatic nitric oxide (NO) detection system
  • In vitro transcription
  • Multiply-primed rolling circle amplification

Azioni biochim/fisiol

Inorganic pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate.
Mg ions are necessary for catalytic activity of crystalline pyrophosphatase.

Qualità

Contaminants: <0.01% ATPase and phosphatases, each (at pH 7 and 4-nitrophenyl phosphate as the substrate).

Stato fisico

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Altre note

For life science research only. Not for use in diagnostic procedures.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

No data available

Punto d’infiammabilità (°C)

No data available


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Yakov Y Woldman et al.
Free radical biology & medicine, 47(10), 1339-1345 (2009-09-16)
Nitric oxide (NO) is a free radical involved in many physiological processes including regulation of blood pressure, immune response, and neurotransmission. However, the measurement of extremely low, in some cases subnanomolar, physiological concentrations of nitric oxide presents an analytical challenge.
Single prokaryotic cell isolation and total transcript amplification protocol for transcriptomic analysis.
Kang Y, et al.
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