03708152001
Roche
Streptavidin Mutein Matrix
suspension, pkg of 5 mL
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352200
NACRES:
NA.21
Prodotti consigliati
Forma fisica
suspension
Confezionamento
pkg of 5 mL
Produttore/marchio commerciale
Roche
Parametri
4-25 °C optimum reaction temp.
Condizioni di spedizione
wet ice
Descrizione generale
Contents
Settled resin of 50% suspension Streptavidin Mutein Matrix.
Settled resin of 50% suspension Streptavidin Mutein Matrix.
Streptavidin mutein, immobilized.
The binding of biotin to avidin/streptavidin is the strongest known noncovalent interaction of biological molecules [(Kd 0.6 x 10-15 M (avidin), 4 x 10-14 M (streptavidin)]. Strong binding provides a great advantage for certain applications of the derived biotin-avidin/streptavidin technology, such as surface immobilization, blotting technologies, immunoassays, cross-linking studies, and histochemistry. However, the use of immobilized avidin/streptavidin for the purification of biotinylated proteins is limited. In this instance, the extreme affinity of the binding partners creates a drawback of enabling elution only under very harsh conditions that usually destroy the functionality of the protein of interest.
Recombinant streptavidin technology was used to screen for a mutant streptavidin with reduced binding affinity toward biotin. By substituting three amino acids, a mutein was obtained with a biotin dissociation constant of 1.3 x 10-7 M.
The Streptavidin Mutein Matrix provides this mutein immobilized onto cross-linked agarose beads in a highly stable and regenerable form, which allows purification of biotinylated proteins that results in excellent purity and recovery.
Recombinant streptavidin technology was used to screen for a mutant streptavidin with reduced binding affinity toward biotin. By substituting three amino acids, a mutein was obtained with a biotin dissociation constant of 1.3 x 10-7 M.
The Streptavidin Mutein Matrix provides this mutein immobilized onto cross-linked agarose beads in a highly stable and regenerable form, which allows purification of biotinylated proteins that results in excellent purity and recovery.
Applicazioni
- Streptavidin Mutein Matrix: Is highly suitable for purification of biotinylated proteins
- Has been tested for use with in vitro expressed biotinylated proteins, using the RTS AviTag Biotinylation Kits
- Is convenient for purification of chemically biotinylated proteins
- It was also used in single-step affinity chromatography and affinity chromatography.
Caratteristiche e vantaggi
- Mild purification conditions: Low biotin dissociation constant allows elution of biotinylated proteins under gentle conditions.
- Stable: No significant loss of binding capacity upon storage. The low dissociation constant of the streptavidin mutein is obtained by genetic engineering and not by dissociation of avidin- or streptavidin tetramers.
- Economic: High binding capacity and multiple reuse allows economic handling of the matrix.
Stoccaggio e stabilità
Store at 2 to 8 °C. (Do not freeze!)
Altre note
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Nancy G Nossal et al.
The Journal of biological chemistry, 282(2), 1098-1108 (2006-11-16)
Our previous electron microscopy of DNA replicated by the bacteriophage T4 proteins showed a single complex at the fork, thought to contain the leading and lagging strand proteins, as well as the protein-covered single-stranded DNA on the lagging strand folded
Dalia Juzumiene et al.
Nuclear receptor signaling, 3, e001-e001 (2006-04-11)
The full-length human androgen receptor with an N-terminal biotin acceptor peptide tag was overexpressed in Spodoptera frugiperda cells in the presence of 1 microM dihydrotestosterone. Site-specific biotinylation of BAP was achieved in vivo by co-expression of E. coli biotin holoenzyme
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.