SCR530

STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

The STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of mouse & human induced pluripotent stem cells.

• Codice UNSPSC: 12352207
• eCl@ss: 32161000
• NACRES: NA.81

Proprietà

• Livello qualitativo: 100
• Produttore/marchio commerciale: STEMCCA
• tecniche: cell culture | stem cell: suitable
• input: sample type induced pluripotent stem cell(s); sample type: mouse embryonic stem cell(s)
• Condizioni di spedizione: dry ice

Descrizione

Descrizione generale

The STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Kit contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of mouse and human induced pluripotent stem cells from mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (HFFs), respectively. The STEMCCA vector is comprised of the transcription factors Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences (Sommer CA, 2009). The use of a single polycistronic lentiviral vector significantly improves reprogramming efficiencies and reduces the number of viral integrations.

Componenti

1. EF1α-STEMCCA (OKSM) Lentivirus: (Part number CS204279) Three (3) vials containing 15 µL of high titer lentivirus.

2. Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50 µL of 10 mg/mL stock.

Qualità

Tested to confirm the generation of iPS cells from p3 mouse embryonic fibroblasts and p6 human foreskin fibroblasts. Other cell types have not been tested and thus similar results can not be guaranteed.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable