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SCM130

Sigma-Aldrich

PluriSTEM® Human ES/iPS Cell Medium

Defined small molecule based serum-free medium that enables feeder-free culture of human ES/iPS cells and allows for media exchanges every other day without compromising the morphology or long term functionality of human pluripotent stem cells.

Sinonimo/i:

Human Embryonic Stem Cell Media, Human iPS Cell Media, Pluripotent Stem Cell Media

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About This Item

Codice UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Livello qualitativo

100

Forma fisica

liquid

Produttore/marchio commerciale

PluriSTEM®

tecniche

cell culture | embryo: suitable
cell culture | stem cell: suitable

Condizioni di spedizione

dry ice

Descrizione generale

EMD Millipore has developed a complete, defined and serum-free medium formulation for the feeder-free culture of human ES and iPS cells. PluriSTEM Human ES/iPS Cell (Cat. No. SCM130) is a small molecule based medium that enables weekend-free culture of human pluripotent stem cells and allows for media exchanges every other day without compromising the morphology or long term functionality of pluripotent stem cells. Pluripotent cells maintained in this moderate feeding regiment exhibited high cell health with minimal spontaneous differentiation,; expressed high levels of pluripotency markers (NANOG, OCT3/4, SOX-2, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), retained differentiation potential and possessed a normal karyotype. PluriSTEM Human ES/iPS Medium is provided as a stand-alone 500 mL bottle that is ready-to-use and does not require additional supplementation. Cells proliferate faster in PluriSTEM as compared to the same cells plated in other serum-free and feeder-free media systems; a typical 4-6 day passage is expected for most pluripotent cell lines.
PluriSTEM has been extensively tested and proven to possess the following characteristics for Human ES/iPS cell culture:

• Supports the culture of pluripotent human ES/iPS cells for greater than 30 passages.
• Eliminates the requirement to feed cells over the weekend. Take the weekend off.
• Eliminates the requirement of daily feeding (i.e. Monday, Wednesday, Friday media exchanges).
• Simple and easy transition of cells to PluriSTEM from feeder-based and feeder-free culture systems.
• Enables superior single cell culture, passage and selection of human pluripotent clones.
• Superior freeze-thaw cell viability and recovery.
• Supports suspension culture.

View our Application Note! (See the Data!)

Qualità

Each lot of PluriSTEM® Human ES/iPS Medium is rigorously quality control tested for the ability to sustain undifferentiated human ES cells for greater than 3 passages.
pH= 7.2-7.4
Osmolality= 315-345
Appearance= Red Clear Liquid

Stoccaggio e stabilità

PluriSTEM Human ES/iPS Medium is provided frozen and can be stored at -20°C until the expiration date on product label. Before use, thaw PluriSTEM at room temperature or overnight at 2-8°C. Do not thaw at 37°C. If desired, PluriSTEM may be aseptically dispensed into working aliquots and stored at -20°C. Use aliquots within expiration date as indicated on label. Thawed aliquots may be stored at 2 – 8°C for up to 2 weeks. Do not refreeze aliquots after thawing. Before use, warm working aliquots to room temperature. Do not warm the medium in a 37°C water bath.

Note legali

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Human pluripotent stem cells on artificial microenvironments: a high content perspective.
Viswanathan, P; Gaskell, T; Moens, N; Culley, OJ; Hansen, D; Gervasio, MK; Yeap, YJ; Danovi, D
Frontiers in Pharmacology null
Doxycycline enhances survival and self-renewal of human pluripotent stem cells.
Chang, MY; Rhee, YH; Yi, SH; Lee, SJ; Kim, RK; Kim, H; Park, CH; Lee, SH
Stem Cell Reports null
Excision of viral reprogramming cassettes by Cre protein transduction enables rapid, robust and efficient derivation of transgene-free human induced pluripotent stem cells.
Kadari, Asifiqbal, et al.
Stem Cell Research & Therapy, 5, 47-47 (2014)

Articoli

A Serum-Free and Feeder-Free Defined Medium That Enables Weekend-Free Culture of Undifferentiated Human ES/iPS Cells

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

HumanKine® Thermostable bFGF, an engineered recombinant protein with enhanced biological functionality on human iPS and neural progenitor cells

Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmental pathways and help regulate mesoderm and ectoderm patterning in the early embryonic development.

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

Robust Differentiation of Human iPSCs into Lineage-Specific Neurons and Glial Cells Utilizing Dual-SMAD Inhibition

Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.

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