S7113
DAPI/Antifade Solution, Ready to Use
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About This Item
Prodotti consigliati
Produttore/marchio commerciale
Chemicon®
Livello qualitativo
Metodo di rivelazione
fluorometric
Condizioni di spedizione
dry ice
Descrizione generale
DAPI: 4′, 6-Diamino-2-phenylindole dihydrochloride. Antifade Solution: p-Phenylenediamine and glycerol in PBS solution. 0.4μg/mL DAPI suspended in Antifade. The product is ready to add to fixed slides or cells cultures.
Applicazioni
A nuclear fluorescent stain used for counterstaining paraffin-embedded, formalin-fixed tissue, metaphase chromosomes, or interphase nuclei that have been hybridized with probes detected with Fluorescein, or equivalent stains. Antifade prevents the permanent loss of fluorescence or fluorescent signal quenching due to photobleaching and prolonged exposure to high intensity light sources.
DAPI binds the minor groove of DNA at AT clusters and binds preferentially to ds DNA (Biochemistry 26:4545, 1987).
Materials Provided
Each vial contains 1 ml of DAPI/Antifade Solution
Warnings and Precautions
DAPI is a potential carcinogen. Antifade is a potential toxin and irritant. Use extreme caution. Avoid contact with eyes, skin and mucous membranes. Use extreme caution. Wear gloves and a lab coat when handling. Follow your institution′s guidelines for hazardous waste disposal. Keep tightly capped and limit exposure to light.
Related Products
Antifade Solution S7114
Propidium Iodide Solution S7109
Propidium Iodide/Antifade Solution S7112
DAPI binds the minor groove of DNA at AT clusters and binds preferentially to ds DNA (Biochemistry 26:4545, 1987).
Materials Provided
Each vial contains 1 ml of DAPI/Antifade Solution
Warnings and Precautions
DAPI is a potential carcinogen. Antifade is a potential toxin and irritant. Use extreme caution. Avoid contact with eyes, skin and mucous membranes. Use extreme caution. Wear gloves and a lab coat when handling. Follow your institution′s guidelines for hazardous waste disposal. Keep tightly capped and limit exposure to light.
Related Products
Antifade Solution S7114
Propidium Iodide Solution S7109
Propidium Iodide/Antifade Solution S7112
Stato fisico
Liquid PBS pH 8.0 containing ~50% glycerol and 0.4μg/mL of DAPI and 2-7mM phenylenediamine.
Stoccaggio e stabilità
Store at -20ºC in undiluted aliquots for up to 1 year from date of receipt. Protect from light
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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