PAT001

Protein A-Turbo - Proximity Biotinylation Enzyme

• Sinonimo/i: ProteinA-Turbo ID
• Codice UNSPSC: 12352202
• NACRES: NA.46

Proprietà

• Stato: liquid
• Livello qualitativo: 200
• Purificato mediante: affinity chromatography
• Confezionamento: pkg of 100 μg
• tecniche: affinity binding assay: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; mass spectrometry (MS): suitable; proximity ligation assay: suitable
• Temperatura di conservazione: -65 to -96°C

Descrizione

Descrizione generale

Protein A Turbo is a recombinantly expressed Protein A-TurboID fusion protein with the capability to bind IgG through the Protein A region, and enzymatically biotinylate proximal proteins non-specifically with the TurboID region.

Specificità

Protein A strongly binds multiple Immunoglobulin G subclasses (IgG1, IgG2, IgG4), but does not bind IgG3. This probe is not compatible with primary antibodies that are IgG3.

Applicazioni

• Proximity protein labeling
• Cell Biology
• Histology
• Fixed cell microscopy
• Tissue staining
• Immunofluorescence
• Liquid Chromatography
• Mass Spectrometry
• Immunoprecipitation

Stato fisico

Purified protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 150 mM imidazole with protease inhibitor cocktail.

Ricostituzione

0.7 mg/mL

Stoccaggio e stabilità

Store at -65°C to -96°C.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H315,H319,H360D
• Consigli di prudenza: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classi di pericolo: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Codice della classe di stoccaggio: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable