MAC133
Anti-Cas9 Antibody, clone 7A9
clone 7A9, from mouse
Sinonimo/i:
dCas9
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
7A9, monoclonal
Reattività contro le specie (prevista in base all’omologia)
all
tecniche
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG1κ
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Descrizione generale
Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1 that is part of that organism’s innate immunity system, or CRISPR (clustered regularly interspaced short palindromic repeat) adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed by 3′-5′ exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or to help distinguish self versus nonself. Researchers hope to exploit the programmability and precise nature of Cas9 cleavage for more effective recombinant DNA technologies.
Specificità
MAC133 recognises both Cas9 and dCas9 (nuclease deficient Cas9)
Immunogeno
N-Terminal domain of Cas9.
Applicazioni
Anti-Cas9 Antibody, clone 7A9 | MAC133 is an antibody against Cas9 Antibody for use in Western Blotting, Immunoprecipitation and Immunocytochemistry.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Secondary Antibodies Adsorbed for Dual Labeling
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Cas9 in 1 µg of Myc-Cas9 expressing HEK293 cell lysate.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).
Qualità
Evaluated by Western Blotting in HEK293 expressing Flag-Cas9 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cas9 in 1 µg of HEK293 cell lysate expressing Flag-Cas9.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cas9 in 1 µg of HEK293 cell lysate expressing Flag-Cas9.
Descrizione del bersaglio
~160 kDa observed
Stato fisico
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Altre note
Concentration: Please refer to lot specific datasheet.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Motore di ricerca dei prodotti.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Maor Sheva et al.
Frontiers in plant science, 11, 607174-607174 (2020-12-17)
While plant cells in suspension are becoming a popular platform for expressing biotherapeutic proteins, the need to pre-engineer these cells to better comply with their role as host cell lines is emerging. Heterologous DNA and selectable markers are used for
Guo-Xiang Ruan et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 25(2), 331-341 (2017-01-23)
As the most common subtype of Leber congenital amaurosis (LCA), LCA10 is a severe retinal dystrophy caused by mutations in the CEP290 gene. The most frequent mutation found in patients with LCA10 is a deep intronic mutation in CEP290 that
Matija Snuderl et al.
Nature communications, 9(1), 2868-2868 (2018-07-22)
Pineoblastoma is a rare and highly aggressive brain cancer of childhood, histologically belonging to the spectrum of primitive neuroectodermal tumors. Patients with germline mutations in DICER1, a ribonuclease involved in microRNA processing, have increased risk of pineoblastoma, but genetic drivers
Garmen Yuen et al.
Nucleic acids research, 45(20), 12039-12053 (2017-10-17)
CRISPR/Cas9 is a powerful gene editing tool for gene knockout studies and functional genomic screens. Successful implementation of CRISPR often requires Cas9 to elicit efficient target knockout in a population of cells. In this study, we investigated the role of
Elif Eren et al.
EMBO reports, 21(11), e50829-e50829 (2020-10-31)
Inflammatory caspase-11 (rodent) and caspases-4/5 (humans) detect the Gram-negative bacterial component LPS within the host cell cytosol, promoting activation of the non-canonical inflammasome. Although non-canonical inflammasome-induced pyroptosis and IL-1-related cytokine release are crucial to mount an efficient immune response against
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.